Detailed view for Tb927.3.1700

Basic information

TDR Targets ID: 18694
Trypanosoma brucei, diacylglycerol acyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.8713 | Length (AA): 490 | MW (Da): 55509 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 2

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03982   Diacylglycerol acyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016747   transferase activity, transferring groups other than amino-acyl groups  

Metabolic Pathways

Glycerolipid metabolism (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127120)

Species Accession Gene Product
Arabidopsis thaliana AT3G51520   diacylglycerol O-acyltransferase 2
Brugia malayi Bm1_55350   diacylglycerol acyltransferase family protein
Candida albicans CaO19.6941   similar to diacylglycerol acyltransferase type 2B
Candida albicans CaO19.14203   similar to diacylglycerol acyltransferase type 2B
Caenorhabditis elegans CELE_W01A11.2   Protein DGTR-1
Caenorhabditis elegans CELE_K07B1.4   Protein K07B1.4, isoform A
Caenorhabditis elegans CELE_F59A1.10   Protein DGAT-2
Cryptosporidium hominis Chro.60038   hypothetical protein
Cryptosporidium parvum cgd6_260   hypothetical protein with 4 transmembrane domains
Dictyostelium discoideum DDB_G0290279   diacylglycerol O-acyltransferase 2
Drosophila melanogaster Dmel_CG1946   CG1946 gene product from transcript CG1946-RA
Drosophila melanogaster Dmel_CG1941   CG1941 gene product from transcript CG1941-RC
Drosophila melanogaster Dmel_CG1942   CG1942 gene product from transcript CG1942-RA
Homo sapiens ENSG00000124003   monoacylglycerol O-acyltransferase 1
Homo sapiens ENSG00000062282   diacylglycerol O-acyltransferase 2
Homo sapiens 80168   monoacylglycerol O-acyltransferase 2
Leishmania braziliensis LbrM.25.2380   diacylglycerol acyltransferase, putative
Leishmania donovani LdBPK_251690.1   diacylglycerol acyltransferase, putative
Leishmania infantum LinJ.25.1690   diacylglycerol acyltransferase, putative
Leishmania major LmjF.25.1630   diacylglycerol acyltransferase, putative
Leishmania mexicana LmxM.25.1630   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_11576   diacylglycerol acyltransferase
Mus musculus ENSMUSG00000052396   monoacylglycerol O-acyltransferase 2
Mus musculus 102632711   2-acylglycerol O-acyltransferase 2-A-like
Mus musculus ENSMUSG00000012187   monoacylglycerol O-acyltransferase 1
Mus musculus ENSMUSG00000030747   diacylglycerol O-acyltransferase 2
Neospora caninum NCLIV_046590   Diacylglycerol acyl transferase, related
Oryza sativa 4340911   Os06g0326700
Oryza sativa 4330511   Os02g0714100
Onchocerca volvulus OVOC7310  
Saccharomyces cerevisiae YOR245C   diacylglycerol O-acyltransferase
Schistosoma japonicum Sjp_0053250   2-acylglycerol O-acyltransferase 2-A, putative
Schistosoma japonicum Sjp_0312950   2-acylglycerol O-acyltransferase 1, putative
Schistosoma mansoni Smp_158510   diacylglycerol O-acyltransferase 1
Schmidtea mediterranea mk4.011671.01  
Schmidtea mediterranea mk4.021164.04  
Schmidtea mediterranea mk4.012113.03  
Schmidtea mediterranea mk4.019233.00  
Trypanosoma brucei gambiense Tbg972.3.1540   diacylglycerol acyltransferase, putative
Trypanosoma brucei Tb927.3.1700   diacylglycerol acyltransferase, putative
Trypanosoma congolense TcIL3000_3_790   diacylglycerol acyltransferase, putative
Trypanosoma cruzi TcCLB.510421.260   diacylglycerol acyltransferase, putative
Trypanosoma cruzi TcCLB.510089.219   diacylglycerol acyltransferase, putative
Toxoplasma gondii TGME49_226370   dgat2l1-prov protein

Essentiality

Tb927.3.1700 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.1700 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.1700 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.1700 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.1700 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_K07B1.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_W01A11.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_W01A11.2 Caenorhabditis elegans sterile wormbase
TGME49_226370 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens diacylglycerol O-acyltransferase 2 Compounds References
Mus musculus monoacylglycerol O-acyltransferase 2 Compounds References
Homo sapiens monoacylglycerol O-acyltransferase 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.1501 0.2887 1
0.2182 1 1
0.1204 1 1
0.0429 1 1
0.0603 1 0.5
0.0098 0.5 0.5
0.0098 0.5 0.5
0.0778 0.6501 1
0.2739 0.5095 1
0.0098 0.5 0.5
0.2732 0.7723 0.7669
0.0818 1 1
0.1702 1 1
0.0511 1 1
0.0479 0.4374 0.4634
0.2652 0.3317 1
0.0098 0.5 0.5
0.2063 1 1
0.3246 1 1
0.5256 0.4765 1
0.0196 0.5 0.5
0.2336 0.3754 1
0.2102 1 1
0.1196 0.3747 0.3214
0.0098 0.5 0.5
0.2885 0.5 0.5
0.187 0.2821 1
0.2739 0.5095 1
0.128 1 1
0.137 1 1
0.0694 0.3088 0.5193
0.2182 1 1
0.2732 0.7723 0.7669
0.1196 0.3747 0.3214
0.2005 0.7257 1
0.0098 0.5 0.5
0.2937 0.5 0.5
0.0333 0.8228 1
0.087 0.5408 1
0.0098 0.5 0.5
0.0909 0.4031 1
0.1742 1 1
0.0282 0.2521 0
0.0267 1 1
0.1253 0.2995 1
0.1883 0.258 1
0.087 0.5408 1
0.0098 0.5 0.5
0.1692 1 1
0.1204 1 1
0.0501 1 1
0.0098 0.5 0.5
0.2807 0.5 0.5
0.1084 0.4036 1
0.3102 0.5 0.5
0.0694 0.3088 0.5193
0.0196 0.5 0.5
0.2132 0.5 0.5
0.2086 1 1
0.2179 0.75 1
0.1204 1 1
0.5256 0.4765 1
0.0916 0.3691 1
0.2179 0.75 1
0.0348 0.5508 1
0.0818 1 1
0.0479 0.4374 0.4634
0.0828 0.3336 1
0.2102 1 1
0.274 1 1
0.0341 0.5 0.5
0.0098 0.5 0.5
0.2739 0.5095 1
0.1241 0.4107 1
0.068 0.3363 1
0.1828 1 1
0.0987 0.2763 1
0.0916 0.3691 1
0.2527 0.3172 1
0.0751 0.5841 1
0.2849 1 1
0.0098 0.5 0.5
0.0098 0.5 0.5
0.2179 0.75 1
0.1245 0.4738 1
0.2575 0.3057 1
0.175 0.3366 0.9295
0.0429 1 1
0.1883 0.258 1
0.1883 0.258 1
0.1613 1 1
0.2807 1 1
0.2179 0.75 1
0.0917 0.2826 1
0.138 1 1
0.068 0.3363 1
0.5256 0.4765 1
0.0098 0.5 0.5
0.2371 0.5 0.5
0.0473 1 1
0.2359 1 1
0.2928 1 1
0.0368 0.9467 0.5
0.3246 1 1
0.0177 0.9585 1
0.3246 1 1
0.1007 0.6696 1
0.0284 1 1
0.2179 0.75 1
0.175 0.3366 0.9295
0.0905 0.3339 1
0.0191 0.8297 1
0.2739 0.5095 1
0.0916 0.3691 1
0.0098 0.5 0.5
0.2729 1 1
0.2148 0.8527 1
0.2835 1 1
0.0852 1 1
0.0098 0.5 0.5
0.0098 0.5 0.5
0.1538 0.2972 1
0.0909 0.4031 1
0.0098 0.5 0.5
0.0282 1 1
0.0833 0.5 0.5
0.154 0.2823 1
0.0372 0.6343 1
0.1501 0.2887 1
0.0098 0.5055 1
0.2297 0.5 0.5
0.0604 0.5 0.5
0.3746 0.2566 1
0.0098 0.5 0.5
0.2005 0.7257 1
0.1166 0.2549 1
0.05 0.521 1
0.0273 0.8286 1
0.0852 1 1
0.1645 0.6358 1
0.0273 0.8286 1
0.0173 1 1
0.1448 1 1
0.1271 1 1
0.1145 1 1
0.0098 0.4143 0.5
0.2527 0.3172 1
0.3246 1 1
0.0098 0.5 0.5
0.2447 0.5 0.5
0.084 1 1
0.1271 1 1
0.1501 0.2887 1
0.2867 1 1
0.1563 0.5 0.5
0.0098 0.4352 0.5
0.0779 0.5064 1
0.2283 1 1
0.0098 0.5 0.5
0.0098 0.3891 1
0.1933 1 1
0.0473 1 1
0.0098 0.5 0.5
0.2126 1 1
0.2575 0.3057 1
0.0098 0.5 0.5
0.0098 0.5 0.5
0.2136 0.2789 1
0.2179 0.75 1
0.2739 0.5095 1
0.1501 0.2887 1
0.2652 0.3317 1
0.212 1 1
0.2185 1 1
0.2181 0.574 1
0.0098 0.5 0.5
0.2795 1 1
0.2807 1 1
0.0176 0.2564 1
0.2394 1 1
0.2259 0.5084 1
0.0098 0.5 0.5
0.0664 0.3309 1
0.2336 0.3754 1
0.0743 1 1
0.0496 0.4057 1
0.0098 0.6539 1
0.3153 1 1
0.2217 1 1
0.068 0.3363 1
0.0098 0.3495 1
0.2652 0.3317 1
0.1204 1 1
0.0098 0.5 0.5
0.0098 0.5 0.5
0.5256 0.4765 1
0.0098 0.5 0.5
0.2148 0.8527 1
0.043 0.5265 1
0.068 0.3363 1
0.3238 0.5 0.5
0.2181 0.574 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.3.1700 (Trypanosoma brucei), diacylglycerol acyltransferase, putative
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